Figure 10.

Knockdown of the P2Y2 receptor attenuates UTP-induced expression changes in EMT-related genes in breast cancer cells. P2Y2-silenced cells (siRNA1 and siRNA2) and control siRNA cells (NC) were treated with or without 100 µM UTP for 12 h (UTP 12 h). Western blot experiments were performed to examine protein levels of (A) Snail and (B) E-cadherin. Expression levels of these proteins were normalized to their respective expression in the control cells (without UTP). Data are presented as mean ± SD (vertical bars). At least 3 independent experiments were performed; *P<0.05.