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. 2017 Nov 7;39(1):138–150. doi: 10.3892/or.2017.6081

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Copyright: © Qiu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 9. — Knockdown of the P2Y2 receptor attenuates ATP-induced expression changes in EMT-related genes in breast cancer cells. P2Y2-silenced cells (siRNA1 and siRNA2) and control siRNA cells (NC) were treated with or without 100 µM ATP for 12 h (ATP 12 h). Western blot experiments were performed to examine protein levels of (A) Snail and (B) E-cadherin. Expression levels of these proteins were normalized to their respective expression in the control cells (without ATP). Data are presented as mean ± SD (vertical bars). At least 3 independent experiments were performed; *P<0.05.