Table II.
Sequences of the primers used in the real-time qPCR experiments.
| Target | Forward primer sequence (5′-3′) | Reverse primer sequence (5′-3′) | Length (bp) |
|---|---|---|---|
| E-cadherin | CTGGGCTGGACCGAGAGA | GAAGGTCAGCAGCTTGAACCA | 60 |
| Claudin-1 | TGAAGTGCTTGGAAGACGATG | GGCAACTAAAATAGCCAGACCT | 95 |
| IL-8 | ACTGAGAGTGATTGAGAGTGGAC | AACCCTCTGCACCCAGTTTTC | 112 |
| Snail | AATCGGAAGCCTAACTACAGCG | GTCCCAGATGAGCATTGGCA | 147 |
| P2Y1 | GTGCTGGTGTGGCTCATTGT | TGGTGTCGTAACAGGTGATGGT | 100 |
| P2Y2 | GCTACAGGTGCCGCTTCAAC | AGACACAGCCAGGTGGAACAT | 170 |
| P2Y4 | GTGAGGTGGAGCTGGACTGTTG | GGTCGGAGGCGGAAGATGA | 136 |
| P2Y6 | CCACAGGCATCCAGCGTAAC | AGGAAGCCGATGACAGTGAGAG | 106 |
| P2Y11 | CCTTCTGTGTCCACCCTCTACTCT | AGTGCTCTTGGCGTCCTCTG | 113 |
| P2Y12 | CACTGCTCTACACTGTCCTGT | AGTGGTCCTGTTCCCAGTTTG | 190 |
| P2Y13 | CACTGCTCTACACTGTCCTGT | AGTGGTCCTGTTCCCAGTTTG | 134 |
| P2Y14 | TGGAGTGTCAGGATGGATATTCT | GTCACCAAGGATCTTGAAAGGAA | 124 |
| β-actin | GGATGCAGAAGGAGATCACTG | CGATCCACACGGAGTACTTG | 90 |