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. 2017 Nov 16;39(1):160–172. doi: 10.3892/or.2017.6100

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Copyright: © Busch et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Effects of etoposide and cisplatin resistance on RB cell line morphology. (A) Images of parental (Ctr), etoposide (Etop) and cisplatin-resistant (CisPt) Y-79, WERI-Rb1 and RB-355 RB cells in culture. (B) Images of parental, etoposide- and cisplatin-resistant RB cell lines after seeding on coverslips and immunocytochemical staining with β-tubulin (green fluorescence) and DAPI counterstaining (blue fluorescence). Morphological comparison revealed that parental controls frequently form chain-like structures, whereas chemoresistant RB cell lines tend to form clusters.