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. 2017 Nov 13;39(1):81–90. doi: 10.3892/or.2017.6096

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Copyright: © Wa et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Downregulation of miR-19a-3p activates TGF-β signaling in PCa cells. (A) Transcriptional activity based on a TGF-β/Smad-responsive luciferase reporter as assessed in the indicated cells. Error bars represent the mean ± SD of three independent experiments. *P<0.05. (B) Western blot analysis showing that upregulation of miR-19a-3p decreased, while downregulation of miR-19a-3p increased nuclear translocation of pSMAD2/3 in PCa cells. The nuclear protein p84 was used as a nuclear protein marker. (C and D) Real-time PCR analysis of downstream bone metastasis-related genes of the TGF-β pathway, including CTGF, PTHRP and IL-11 in the indicated cells. Transcript levels were normalized to GAPDH expression. Error bars represent the mean ± SD of three independent experiments. *P<0.05.