Video 10. Phosphomimetic S96D allele of Mira tagged with mCherry at the C-terminus.

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DOI: 10.7554/eLife.29939.019

S96D localizes to microtubules in interphase, but is asymmetric in mitosis. Note the signal resembling subcortical microtubules in interphase converging at the apical pole. After NEB a basal crescent is detectable. At 115:30 a z-stack spanning the entire NB was collected and the maximum projection is frozen. After this, 50 µM colcemid was added to reveal if Mira^S96D::mCherry binds to the cortex. Next frozen frame: similar stack after 30 min in colcemid. Next frozen frame: 50 min in colcemid – no cortical signal is detectable. Last frozen frame 65 min in colcemid. Time stamp: mm:ss. Scale 15 µm.