Video 14. 200 µM Y-27632 induced uniform cortical Mira in mitosis localizes independently of an intact actin network.
A Baz::GFP and Mira::mCherry expressing NB was cultured in the presence of 200 µM Y-27632 and then arrested with colcemid. 5 µM LatA was added after the first frame of the movie. LatA induces loss of Baz asymmetry, yet Mira remains cortical. Z-stacks shown. Z stacks taken every 2 min. Time stamp: hh:mm.