Video 8. Control mira^mCherry allele generated by CrispR/Cas9.

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DOI: 10.7554/eLife.29939.017

Mira localizes to the interphase cortex, from where it is cleared before NEB. Then Mira relocalizes to a larger crescent. Therefore this allele and Mira::mCherry (BAC rescue) are undistinguishable in terms of Mira dynamics. This control further shows that the MS2 binding site in the BAC rescue construct does not interfere with Mira cortical dynamics. Time stamp: hh:mm.