Figure 5.

The expression of LASP1 is negatively regulated by miR-133a in breast cancer cells. (A) Real-time PCR and (B) western blot analysis were used to examine the mRNA and protein expression of LASP1 in SK-BR-3 and MDA-MB-231 cells transfected with miR-133a mimic or scramble miR mimic as NC group. SK-BR-3 and MDA-MB-231 cells were then transfected with NC inhibitor or miR-133a inhibitor, respectively. (C) Real-time PCR was used to examine the miR-133a levels. (D) Real-time PCR and (E) western blot analysis were used to examine the mRNA and protein expression of LASP1. For A and B, **P<0.01 vs. miR-NC. For C-E, **P<0.01 vs. NC inhibitor. NC, negative control.