Table I.
The TaqMan stem-loop primers for reverse transcription PCR and the forward and reverse primers for real-time PCR.
| Accession | ID | Sequence | |
|---|---|---|---|
| MIMAT0000267 | hsa-miR-210 | Sequence | CUGUGCGUGUGACAGCGGCUGA |
| TaqMan primer | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTCAGCC | ||
| PCR-F | CTGTGCGTGTGACAGC | ||
| MIMAT0000758 | hsa-miR-135b | Sequence | UAUGGCUUUUCAUUCCUAUGUGA |
| TaqMan primer | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTCACAT | ||
| PCR-F | TATGGCTTGTCATTCCT | ||
| MIMAT0002876 | hsa-miR-505 | Sequence | CGUCAACACUUGCUGGUUUCCU |
| TaqMan primer | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACAGGAAA | ||
| PCR-F | CGTCAACACTTGCTGG | ||
| MIMAT0000069 | hsa-miR-16 | Sequence | UAGCAGCACGUAAAUAUUGGCG |
| TaqMan primer | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACCGCCAA | ||
| PCR-F | GGGTAGCAGCACGTAAATAT | ||
| U6 | PCR-F | GCTCGCTTCGGCAGCACA | |
| PCR-R | AACGCTTCACGAATTTGCGT | ||
| GAPDH | PCR-F | ACCCAGAAGACTGTGGATGG | |
| PCR-R | CAGTGAGCTTCCCGTTCAG | ||
| HMGB1 | PCR-F | CTTCTTGAGGGGAAGCTAGT | |
| PCR-R | TTTTGGATGTTCAGTTATGG |