Figure 3.

miR-208b targets H2AX and represses H2AX expression by binding to its 3′-UTR. (A) The 3′-UTR region of H2AX contains a sequence that matches the specific binding sequences of miR-328-3p. (B) The targeting effect of miR-328-3p on H2AX 3′-UTR was detected by a luciferase reporter assay in HOS and U2OS cells. (C) The expression levels of H2AX in HOS and HOS-2R cells were determined by western blotting. (D) The expression levels of H2AX in HOS and U2OS cells were determined by western blotting after overexpressing or silencing miR-328-3p. *p<0.05.