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H2AX downregulation reduces radioresistance in osteosarcoma cells. (A) To silence H2AX, si-H2AX was used, and the knockout efficiency of H2AX was detected by western blotting. (B) The survival rate of osteosarcoma cells with or without H2AX silencing after irradiation at 2 Gy. (C) The apoptosis assay was performed by flow cytometry following 2 Gy X-ray irradiation. (D) Cell viability assay was performed in si-control and si-H2AX cells after 2 Gy X-ray irradiation. *p<0.05.
