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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Blood Cells Mol Dis. 2017 Nov 29;69:1–9. doi: 10.1016/j.bcmd.2017.11.003

Figure 6. Hemoglobin expression during erythroid differentiation of HUDEP-2 cells with knockdown of HMI-LNCRNA.

Figure 6

(A) Illustration of timeline for infection and culture of HUDEP-2 cells. Cells were maintained in expansion medium for 2 weeks after transduction, and then placed in differentiation medium for up to 7 days. Doxycycline (DOX) was removed at Day 5 to promote erythroid maturation. (B) Relative quantity for HBG and HBB transcripts were analyzed by qPCR in naïve HUDEP-2 cells (n=3), and cells transduced with scrambled shRNA (n=3) and HMI-lncRNA shRNA (n=3) at Day 0, 5 and 7 of differentiation. (C) Percent hemoglobin of HBG to HBB was determined by qPCR. For all qPCR analyses, ACTB was used as the endogenous control. p-values: *< 0.05; **< 0.005; ***<0.0005; NS (not significant). p-values were obtained by Student T-test.