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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Blood Cells Mol Dis. 2017 Nov 29;69:1–9. doi: 10.1016/j.bcmd.2017.11.003

Figure 7. The effects of knocking down HMI-LNCRNA on erythroid maturation of HUDEP-2 cells.

Figure 7

Naïve HUDEP-2 cells, and cells transduced with scrambled shRNA and HMI-lncRNA shRNA (cultured in expansion medium) were stained with PE-labeled transferrin receptor (CD71) and PerCP/Cy5.5-labeled glycophorin-A (CD235) antibodies, and analyzed by flow cytometry to discriminate between cells that are positive and negative for GFP, CD71 and CD235. Data was analyzed with FlowJo. Average percent GFP+, CD71+/CD235- and CD71+/CD235+ for each group was plotted on a bar graph. p-values: *< 0.05; **< 0.005; ***<0.0005; NS (not significant). p-values were obtained by Student T-test.