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. Author manuscript; available in PMC: 2018 Dec 11.
Published in final edited form as: Hypertension. 2017 Sep;70(3):634–644. doi: 10.1161/HYPERTENSIONAHA.117.09016

Figure 3. NO2− signaling and methemoglobin and 15NO-Hb formation.

Figure 3

(A) Following oral 15NO3−, no significant increase in methemoglobin (MetHb) occurs. (B) Following oral 15NO2−, a significant rise in MetHb occurs through 1 hr. (C) A representative EPR spectra (red) shows the characteristic hyperfine splitting of 15NO-Hb from one subject following oral 15NO2− (raw tracings shown in blue). The fit is composed of 42% pentacoordinate 15N alphanitrosyl Hb, 21% hexacoordinate alphanitrosyl Hb, and 38% betanitrosyl Hb. Inclusion of pentacoordinate 14N alphanitrosyl Hb does not substantially improve the fit (Fig. S3B). (D) 15NO-Hb formation is detected with oral 15NO2− treatment only at 0.5 to 1 hours, with no 15NO-Hb detected with oral 15NO3− treatment. NS = not significant, N.D. = not detected. Repeated measures ANOVA with time as the within-subject effect was used to evaluate response to drug treatment for the endpoint measures in A–B.