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. 2017 Aug 11;32(2):462–469. doi: 10.1038/leu.2017.233

Figure 2.

Figure 2

The JAK2V617F mutation alters vascular niche function. (a) Representative flow cytometry density and histogram plots for marrow ECs. (b) Total marrow EC, sinusoidal marrow EC, and arterial marrow EC number in Tie2/FF1 recipients (red bar, n=5) and WT recipients (green bar, n=5). (c, d) The expression levels of CXCL12 and SCF in pooled arterial (c) and sinusoidal (d) marrow ECs of Tie2/FF1 recipients (red bar, n=5) and WT recipients (green bar, n=5) were measured using real-time qPCR. Gene expression in Tie2/FF1 marrow ECs is shown as the fold-change compared with the WT marrow EC expression which was set as ‘1’. (e, f) Representative histogram plots and flow cytometry quantitative analysis of intracellular CXCL12 (e) and membranal SCF (f) expression in marrow ECs of Tie2/FF1 recipients (red) and WT recipients (green) (n=3-5). (g, h) Representative histogram plots and flow cytometry quantitative analysis of membranal CXCR4 (g) and cKIT (h) expression in JAK2WT HSPCs (gray bar) and JAK2V617F HSPCs (black bar) in the Tie2/FF1 recipients (n=4).