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. 2017 Nov 21;22(2):1247–1256. doi: 10.1111/jcmm.13403

Figure 1.

Figure 1

Effects of DHM on cell viability, adipogenesis and glucose uptake in 3T3‐L1 cells. 3T3‐L1 cells were treated with vehicle or differentiation medium for 8 days. ROSI (10 μM) or DHM (1, 3 or 10 μM) was added to the differentiation medium at the beginning of differentiation. A Cell viability was analysed using CCK‐8 kit. Data represent the mean ± S.E.M. of eight independent experiments. a P < 0.05, b P < 0.01 versus vehicle. B Glucose uptake was detected by 2‐NBDG uptake assay. Data are presented as the mean ± S.E.M. of five independent experiments. a P < 0.05, b P < 0.01 versus vehicle. C Lipid accumulation was visualized by Oil Red O staining (magnification: ×200). D Quantification of lipid drop dissolved in isopropanol with detection at 510 nm. Data are presented as the mean ± S.E.M. of five independent experiments. b P < 0.01 versus vehicle; *P < 0.05, **P < 0.01 versus differentiated control. E Protein expression of FABP4 determined by Western blot analysis.