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. 2017 Nov 6;22(2):1302–1315. doi: 10.1111/jcmm.13431

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© 2017 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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ALP staining and osteogenesis‐related gene expression in bone marrow mesenchymal stem cells induced in CM‐based osteogenic medium for 7 days; Norm was used as a control. (A) General view of ALP staining and quantitative analysis of ALP activity. Relative mRNA expression levels (normalized to β‐actin) of osteogenesis‐related genes: (B) Runx2, (C) ALP, (D) SP7, (E) OCN, and (F) COL‐1. Data are expressed as the mean ± SD; *P < 0.05, **P < 0.01 and ***P < 0.001 represent significant differences between the indicated columns.