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. 2017 Nov 6;22(2):1302–1315. doi: 10.1111/jcmm.13431

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© 2017 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Effects of CMs generated by different Mφ phenotypes on cell sheet formation by bone marrow mesenchymal stem cells; Norm was used as a control. (A) Morphologies of cell sheets observed by HE staining following 7 days of sheet induction. Scale bar: 10 μm. (B) Representative images of the obtained cell sheets (general view) and quantification of cell sheet thickness using Adobe Photoshop CS5. Relative mRNA levels of extracellular components in the cell sheets (normalized to β‐actin): (C) fibronectin, (D) COL‐1 and (E) integrin β1. Data are expressed as the mean ± SD; *P < 0.05, **P < 0.01 and ***P < 0.001 represent significant differences between the indicated columns.