Table 1.
Primer sequences used in this study
| Gene | Full name | Primer | Sequences (5′‐3′) |
|---|---|---|---|
| iNOS | Inducible nitric oxide synthase | Forward | CAAGCTGAACTTGAGCGAGGA |
| Reverse | TTTACTCAGTGCCAGAAGCTGGA | ||
| TNF‐α | Tumour necrosis factor‐α | Forward | TATGGCCCAGACCCTCACA |
| Reverse | GGAGTAGACAAGGTACAACCCATC | ||
| CCR7 | Chemokine receptor type‐7 | Forward | TGGTCAGTGCCCAAGTGGAG |
| Reverse | TCAAAGTTGCGTGCCTGGAG | ||
| Arg‐1 | Arginine‐1 | Forward | AGCTCTGGGAATCTGCATGG |
| Reverse | ATGTACACGATGTCTTTGGCAGATA | ||
| CD206 | CD206 | Forward | AGCTTCATCTTCGGGCCTTTG |
| Reverse | GGTGACCACTCCTGCTGCTTTAG | ||
| IL‐10 | Interleukin‐10 | Forward | GCCAGAGCCACATGCTCCTA |
| Reverse | GATAAGGCTTGGCAACCCAAGTAA | ||
| Adiponectin | Adiponectin | Forward | GAGAGAAAGGAGATGCAGGT |
| Reverse | GAACGCTGAGCGATACACAT | ||
| PPAR‐γ | Peroxisome proliferator activated receptor‐γ2 | Forward | AGCTCCAAGAATACCAAAGT |
| Reverse | ACCCTTGCATCCTTCACAAG | ||
| Runx2 | Runt‐related transcription factor‐2 | Forward | GACTGTGGTTACCGTCATGGC |
| Reverse | ACTTGGTTTTTCATAACAGCGGA | ||
| ALP | Alkaline phosphatase | Forward | CTTCTTGCTGGTGGAAGGA |
| Reverse | AAAACGTGGGAATGATCAGC | ||
| SP7 | SP7 transcription factor‐2 | Forward | GGGGAAAGGAGGCACAAAG |
| Reverse | GTGAGGGAAGGGTGGGTAGTC | ||
| OCN | Osteocalcin | Forward | CTGACAAAGCCTTCATGTCCAA |
| Reverse | GCGCCGGAGTCTGTTCACTA | ||
| COL‐1 | Collagen‐1 | Forward | GCTGGAGTTTCCGTGCCT |
| Reverse | GACCTCGGGGACCCATTG | ||
| Fibronectin | Fibronectin | Forward | AATCACAGTAGTTGCGGCAGGAGA |
| Reverse | TCTGTCCCAGGCAGGAGATTTGTT | ||
| Integrin β1 | Integrin β1 | Forward | CGCAGAACAATAGGTGCTGAAATTAC |
| Reverse | TGACACTGAGAACCACAAACGGC | ||
| β‐actin | β‐actin | Forward | CTCTTTTCCAGCCTTCCTTCTT |
| Reverse | GAGGTCTTTACGGATGTCAACG |