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RT‐PCR was used to measure the mRNA expression levels of BSP, OC and Runx2 of cells cultured in different media after 7 and 14 days. Expressions of OC and Runx2, both significantly increased in PRF group, compared with those exposed to the control group at both 7 and 14 days (A, B). The mRNA expression level of BSP was significantly increased in the cells cultured in the PRF medium at 14 days (C). *Statistically significant difference compared to control and PRF group (P < 0.05).
