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. 2018 Jan 24;22(2):1047–1055. doi: 10.1111/jcmm.13461

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© 2017 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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(A) Western blot analysis was used to measure the protein expression levels of BSP, OC and Runx2 of the cells cultured in different media after 7 and 21 days. The results showed that the protein expression levels of OC and Runx2 were greatly increased in the cells grown in the PRF media at 7 days and those of BSP, OC and Runx2 were all increased at 21 days. (B) ALP activity measurement showed higher ALP activities of rat PDLSCs in the PRF group than those in the control group at 7, 12, 23 and 30 days. *Statistically significant difference compared to control and PRF group (P < 0.05).