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RT‐PCR was performed to measure the mRNA expression levels of COL1A, Opn and RUNX2 in different groups. All bone markers showed higher expression levels at 24 days than at 12 days. In addition, the PRF + cells group showed the highest mRNA expression levels of Col1a, Opn and RUNX2 at both 12 and 24 days (A, B, C). *Statistically significant difference (P < 0.05).
