Table 1.

List of primers used in this study

PCR	Primer	Sequence (5′‐3′)	Product size (bp)	PCR cycles	Annealing temperature (°)
RT‐PCR	ADAMTS9‐F	CATGCAGTTTGTATCCTG	218	32	55
	ADAMTS9‐R	GCGTTCTTTTGAAGTGGACG
	GAPDH‐F	TCCTGTGGCATCCACGAAACT	315	23	55
	GAPDH‐R	GAAGCATTTGCGGTGGACGAT
qRT‐PCR	p53‐F	TCAACAAGATGTTTTGCCAACTG	118	40	60
	p53‐R	ATGTGCTGTGACTGCTTGTAGATG
	Ecad‐F	TGCCCAGAAAATGAAAAAGG	200	40	60
	Ecad‐R	GTGTATGTGGCAATGCGTTC
	VIM‐F	GACCAGCTAACCAACGACAA	150	40	60
	VIM‐R	GTCAACATCCTGTCTGAAAGAT
	SNAIL‐F	GAGGCGGTGGCAGACTAG	159	40	60
	SNAIL‐R	GACACATCGGTCAGACCAG
	VEGFA‐F	CACACAGGATGGCTTGAAGA	136	40	60
	VEGFA‐R	AGGGCAGAATCATCACGAAG
	TGFβ1‐F	AATTGAGGGCTTTCGCCTTAG	87	40	60
	TGFβ1‐R	CCGGTAGTGAACCCGTTGAT
	TGFβR1‐F	ATGGGCTCTGCTTTGTCTCT	254	40	60
	TGFβR1‐R	AGCAATGACAGCTGCCAGTT
	EGF‐F	GCTGCTCACTCTTATCATTCTG	234	40	60
	EGF‐R	CATGATCACTGAGACACCAG
	EGFR‐F	TTCCTATGCCTTAGCAGTCTTAT	142	40	60
	EGFR‐R	GATGCTCTCCACGTTGCACAG
	β‐actin‐F	GTCTTCCCCTCCATCGTG	113	40	60
	β‐actin‐R	AGGGTGAGGATGCCTCTCTT
MSP	ADAMTS9‐M1	TTTTTCGTTTTTTTTTGTTCGTTC	114	40	60
	ADAMTS9‐M2	AAACTAAACCGCTCGAACCG
	ADAMTS9‐U1	GTTTTTTGTTTTTTTTTGTTTGTTT	117	40	58
	ADAMTS9‐U2	AAAAACTAAACCACTCAAACCA
BGS	ADAMTS9‐BGS1	GTATTTGAGAGGTTGTGGATT	390	40	60
	ADAMTS9‐BGS2	CCTCCTACCCTCCTTAACTA