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. 2017 Oct 5;22(2):834–848. doi: 10.1111/jcmm.13365

Figure 2.

Figure 2

Western blotting of TAT‐MTScs‐FXN processing. (A) Cells from Scr, Fxn1 and Fxn2 treated with 7 μg/ml TAT‐MTScs‐FXN 12 hrs after lentiviral transduction were lysed after 5 days of culture and blotted to detect frataxin levels (β‐actin was used for normalization). Results indicate penetration and processing of TAT‐MTScs‐FXN in all cultures. Differences in intermediate forms are evident in Fxn1 and Fxn2 compared to control (Scr) conditions. The lane on the right is to show the molecular weight of the intact TAT‐MTScs‐frataxin form. Lane M indicates the position of the molecular weight marker of 25 kD. (B) The histogram illustrates the relative amounts of frataxin forms when TAT‐MTScs‐frataxin (7 μg/ml) was added to the cultures 12 hrs after lentivirus withdrawal. Error bars represent mean ± S.E.M., n = 4.