Figure 3.

Enforced TMEM207 expression abrogates the binding of WWOX to HIF‐1α (A) Representative result of immunoprecipitation of TMEM207‐expressing and control SAS cells with anti‐WWOX, followed by Western immunoblotting with anti‐HIF‐1α. Notably, the HIF‐1α protein band is only present in the immunoprecipitates of the control cells. (B) Representative Western immunoblot of lysates of SAS cells harbouring the TMEM207 expression vector or control cells. The TMEM207‐expressing cells (labelled ‘TMEM207′) exhibited detectable levels of HIF‐1α, even under normoxic conditions, whereas a weak or no HIF‐1ɑ band was detected in control SAS cells (labelled ‘Mock’) under normoxic conditions. These experiments were repeated using different clones, and consistent results were obtained.