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. 2017 Oct 9;22(2):1081–1094. doi: 10.1111/jcmm.13358

Figure 1.

Figure 1

Dimethyl fumarate (DMF) pre‐treatment protected human SHSY5Y neuronal cells by Aβ1‐42‐induced cell death. Cell death was assessed 24 hrs after incubation with the indicated concentrations of DMF (1‐10‐30‐50‐100 μM), underlying that only DMF 1‐10‐30 μM lacked cytotoxicity (A). Stimulation of cells with Aβ1‐42 1 μM significantly reduced viability compared to the control group and only pre‐treatment with DMF 30 μM significantly limited this cell death (B). MTT assay compared DMF and MMF both at the dose of 30 uM. MMF reduced partially cell death, while DMF confirmed having the greatest effect to preserve cell viability (C). Averages for MTT: 84,6% DMF versus 47,6% Aβ1‐42; 62,6% MMF versus 47,6% Aβ1‐42. Also, DMF 30 μM pre‐treatment determined a considerable reduction in the Aβ1‐42 amount, in cells 24 hrs after Aβ stimulation (D). Data are representative of at least three independent experiments. (A)***P < 0.001 versus Ctr; (B) ***P < 0.001 versus Ctr and ### P < 0.001 versus Aβ1‐42; (C) ***P < 0.001 versus Ctr, ## P < 0.01 versus Aβ1‐42 and ### P < 0,001 versus Aβ1‐42; (D) ***P 0.001 versus Ctr and ### P < 0.001 versus Aβ1‐42.