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. 2017 Oct 9;22(2):1081–1094. doi: 10.1111/jcmm.13358

Figure 7.

Figure 7

Effect of DMF in SHSY5Y following Nrf2 siRNA knockdown. Nrf2 mRNA expression in mouse SH‐SY5Y that were either transfected for 48 hrs with 20 nM control siRNA and 20 nM Nrf2‐specific siRNA, and treated with transfection reagent (TR) only or untreated cells (CTRL) was determined using real‐time PCR. Values are normalized to GAPDH and expressed as fold change to untreated control cells (A). The lack of Nrf2 significantly abolished DMF protective effect, increasing SH‐SY5Y susceptibility to Aβ1‐42 damage; in fact, the pre‐treatment with DMF was not able to preserve from Aβ1‐42‐induced cell death (54% and 49%, respectively) (B); moreover, the pre‐treatment with DMF did not protect from intracellular ROS production (D) and phosphorylation of tau protein (C). Western blot analysis has demonstrated a lost of anti‐inflammatory effect of DMF showing an Nf‐kB expression compared to Aβ1‐42 group (E). Data are representative of at least three independent experiments. (A) **P < 0.01 versus CTRL, TR and con siRNA, (B) ***P < 0.001 versus Ctr; (C) ***P < 0.001 versus Ctr; (D) ***P < 0.001 versus Ctr; (E) ***P < 0.001 versus Ctr and *P < 0,05 versus Ctr.