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. 2017 Oct 20;22(2):1236–1246. doi: 10.1111/jcmm.13401

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© 2017 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Analysis of AQP4 pools by BN‐PAGE. (A, B) AQP4 immunoblot of nSEC fractions from both whole brain (A) and skeletal muscle (B), after a 3–9% gradient BN gel. Note the presence of several AQP4 pools and the different elution profile of AQP4 in the two tissues. Depending on their position on the blot, AQP4 pools were classified as high, medium or low molecular weight pools. (C) Densitometry analysis of the BN‐PAGE immunoblot results to evaluate the relative abundance of the three AQP4 pools in the two tissues (*P < 0.01, n = 3. anova test). Similar protein loading in each lane was evaluated by Coomassie blue staining of the PVDF membrane.