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. 2018 Jan 24;38(4):843–857. doi: 10.1523/JNEUROSCI.2658-17.2017

Figure 8.

Figure 8.

ARF6 and ARF6-GTP during hair bundle development. A, Immunoblot detection of ARF6. Total ARF6 levels are constant during postnatal utricle development, and are unchanged in rda/rda hair cells. Blot was probed separately for actin (below). B–G, ARF6 and ARF6-GTP immunolabeling in profile views of C57BL/6 utricles during development. Little change in ARF6, but ARF6-GTP is elevated in some hair cells at P8. Panel sizes: 30 × 30 μm. H–M, ARF6 and ARF6-GTP immunolabeling in cross-sections of C57BL/6 utricles at the cell apex (cuticular-plate level) during development. Arrows indicate cells with higher levels of ARF6-GTP. Panel sizes: 100 × 100 μm. N, Immunocytochemistry quantitation of ARF6 levels relative to phalloidin. O, Immunocytochemistry quantitation of ARF6-GTP levels relative to phalloidin. ***p < 0.001; **p < 0.01; ns, Not significant.