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. 2017 Dec 15;4(1):55–68. doi: 10.1002/cjp2.88

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© 2017 The Authors The Journal of Pathology: Clinical Research published by The Pathological Society of Great Britain and Ireland and John Wiley & Sons Ltd

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Immunostaining of specimens from breast cancer patients. Mucinous, grade 2 breast cancer of a 45‐year‐old female patient (ER positive, PR weakly positive, Her2 negative; (A) HE stain of the primary carcinoma (×10 objective magnification); (B) hypo‐BSP stain of the primary carcinoma with rat monoclonal antibody IDK‐1 (×20 objective magnification) showing moderate local expression; (C) HE stain of metachronous (7 years) spinal metastasis (thoracic vertebrae 3 + 4, ×4 objective magnification); (D) hypo‐BSP stain of a metachronous (7 years) spinal metastasis with rat monoclonal antibody IDK1 (×40 magnification) showing diffuse distribution of moderate cytoplasmic hypo‐BSP expression. Invasive, grade 2 ductal breast cancer of a 47‐year‐old female patient (ER strongly positive, PR strongly positive, Her2 negative; (E) HE stain of the primary carcinoma (×4 objective magnification); (F) hypoglycosylated BSP stain of the primary carcinoma with rat monoclonal antibody IDK1 (×4 objective magnification) showing focal, moderate cytoplasmic expression; (G) HE stain of a synchronous spinal metastasis (thoracic vertebra 6, ×20 objective magnification); (H) hypoglycosylated BSP stain of a synchronous spinal metastasis with rat monoclonal antibody IDK1 (×20 objective magnification) showing diffuse distribution of moderate to strong cytoplasmic expression.