Table 3.

Experimentally detected unique peptides of the phycobiliproteins of G. theta

Protein	Start and end in precursor	Start and end in predicted mature protein	Unique tryptic peptide	Highest peptide score	Lowest peptide expectation value	Spectra with expectation values <0.05
Beta		1–7	MLDAFSR (N-terminus)	47.64	1.70E−05	14
		16–28	AAYVGGADLQALK	117.63	1.70E−12	90
		16–29	AAYVGGADLQALKK	90.98	8.10E−10	147
		85–91	DGEIILR	39.35	0.00012	6
		92–108	YVSYALLSGDSSVLEDR	155.02	3.20E−16	289
		115–129	ETYSSLGVPANSNAR	120	1.00E−12	230
		130–149	AVSIMKACAVAFINNTASQR	145.61	2.80E−15	30
		136–149	ACAVAFINNTASQR	155.02	3.20E−16	160
		150–171	KLSTPQGDCSGLASECASYFDK	114.82	3.30E−12	31
		151–171	LSTPQGDCSGLASECASYFDK	155.02	3.20E−16	26
GtcpeA1	42–51	9–18	APVITVFDHR	73.05	5.00E−08	181
GtcpeA2	59–68	1–10	IPLGTQIAGK (N-terminus)	25.67	0.0027	30
	75–88	17–30	ANFYAPEVTIFDHR	76.29	2.40E−08	6
	102–112	44–54	TGDQDDEMLVR	89.35	1.20E−09	10
	118–127	60–69	VFCPEAEAAK	13.82	0.041	1
	128–137	70–79	VLASTLSVLK	77.34	1.80E−08	39
GtcpeA3	45–54	9–19	APVVTVFDHR	91.3	7.40E−10	35
	62–80	26–44	NTEYQGLPANSQDDEMLVK	155.02	3.20E−16	90
	61–80	25–44	RNTEYQGLPANSQDDEMLVK	18.77	0.013	1
	86–105	50–69	VAINEAAATDLVQQLLGTLK	120.64	8.60E−13	16
	86–106	50–70	VAINEAAATDLVQQLLGTLKK (C-terminus)	106.65	2.20E−11	13
GtcpeA4	43–55	6–18	SNTAPIVQIFDHR	92.01	6.30E−10	35
	84–104	47–67	VIPAPEALAASVLQVTVGNLK	93.35	4.60E−10	12
GtcpeA5	63–74	6–17	AIAPVITIFDHR	112.95	5.10E−12	20
	88–98	31–41	ANTYDDYMLVK	58.6	1.40E−06	16
	87–98	30–41	KANTYDDYMLVK	58.06	1.60E−06	8
	101–124	44–52	GEVITVSTSTAASVLAETFGLLDR	37.6	0.00017	1
	99–124	42–67	VKGEVITVSTSTAASVLAETFGLLDR	141.73	6.70E−15	32
GtcpeA6	78–89	9–20	GFLAPQSYQSYR	91.23	7.50E−10	120
	90–106	21–37	KGQLLNIAPVIQVHDER	55.2	3.00E−06	16
	91–106	22–37	GQLLNIAPVIQVHDER	97.83	1.60E−10	16
	120–132	51–63	VGTEDDAMCVSVK	132.58	5.50E−14	174
	135–161	66–92	AIPANTNLAASILSDFQIYCQDGSCPK (C-terminus)	126.81	2.10E−13	129
GtcpeA8	77–90	25–38	EYVGPASGDEDDNR	76.52	2.20E−08	6
	99–117	47–65	ITVSETEAAAALQEFISYR	154.66	3.40E−16	41
GtcpeA9	98–107	48–57	IAVSEGDAAK	26.6	0.0022	3
GtcpeA10	34–46	2–14	AAGYSTASPYSSK (N-terminus)	28.85	0.0013	3
	47–64	15–32	NSNMGPAFAPVITIFDNR	155.02	3.20E−16	71
	79–89	47–57	AGDENDEMLVK	28.24	0.0015	10
	95–107	63–75	IPFPTDDVVNEFR	145.05	3.10E−15	50
	95–108	63–76	IPFPTDDVVNEFRR	25.22	0.003	7
	109–120	77–88	ENLAIQGNLDLR	67.25	1.90E−07	18
	109–130	77–98	ENLAIQGNLDLRAPQITIFDHR	26.91	0.002	3
	121–130	89–98	APQITIFDHR	125.77	2.60E−13	79
	144–154	112–122	AGTYDDEMLVK	32.63	0.00055	29
	166–179	134–147	LAQQVLEQTIGVLK	84.23	3.80E−09	29
	166–181	134–149	LAQQVLEQTIGVLKAK (C-terminus)	31.42	0.00072	1
GtcpeA11	59–68	8–17	APEITIFDHR	89.96	1.00E−09	61
	82–92	31–41	AGTSDDEMCVK	42.6	5.50E−05	10
	98–107	47–56	VEASLTLAEK	27.29	0.0019	25
	119–129	68–78	GIDGDYTGVVK	70.2	9.50E−08	55
GtcpeA12	64–79	8–23	VQTQSSFGVINDNMGK	129.58	1.10E−13	51
	80–91	24–35	GAMPVITVFDHR	67.79	1.70E−07	14
	105–115	49–59	SGDQDDEMCVK	32.64	0.00054	6
	120–129	64–73	TPYTYPQYTR	52.09	6.20E−06	52
	130–145	74–89	QATALLNEIIGVTYTK	155.02	3.20E−16	50
GtcpeA13	79–90	18–29	NFAPVITVFDHR	66.65	2.20E−07	11
	105–115	44–54	SNDENDEMLVK	18.13	0.015	2
	141–149	80–88	GTFYQYPIR	64.48	3.60E−07	32
GtcpeA14	67–84	1–18	IGEGPSNTFSGQNGIATK (N-terminus)	131.28	7.50E−14	65
	85–99	19–33	TANGLLGTSIVGVGK	146.89	2.00E−15	46
	107–118	41–52	NFAPMITIFDAR	95.41	2.90E−10	43
	132–142	66–76	AGTTDDEMLVK	28.18	0.0015	1
	150–160	84–94	VPEYQAAAFTR	73.4	4.60E−08	51
	161–171	95–105	EQLGYTYPTTR	57.5	1.80E−06	55
GtcpeA15	78–89	17–28	NAAPVITIFDHR	96.09	2.50E−10	55
	98–114	37–53	EYTGAPSNDYNDEMLVK	155.02	3.20E−16	60
GtcpeA16	66–81	4–19	MPADSYTPIITIFDAR	155.02	3.20E−16	96
	86–105	24–43	GAGEYEGSPAGDNNDEMAVK	155.81	2.60E−16	186
	111–122	49–60	LEPDTGFAEQIK	57.2	1.90E−06	7
	110–122	48–60	KLEPDTGFAEQIK	88.18	1.50E−09	31
	111–131	49–69	LEPDTGFAEQIKAETLGLMSK	19.97	0.01	1
GtcpeA17	44–55	8–19	GVAPIITVFDHR	44.81	3.30E−05	3
	60–81	24–45	GKADSEYQGALANGPEDEMLVK	28.48	0.0014	3
	62–81	26–45	ADSEYQGALANGPEDEMLVK	154.68	3.40E−16	31
	87–96	51–60	VPLSAGFADK	35.26	0.0003	3
	97–113	61–77	VLQQTLGTFGQAPAGAK	155.02	3.20E−16	21
GtcpeA18	59–75	4–20	TMPADGYAPVITVFDHR	113.61	4.40E−12	81
	89–99	34–44	SNDMEDGMLVK	54.99	3.20E−06	54
	107–126	52–71	VTESYAASLLQEQLGYINDK	153.61	4.40E−16	60
	107–127	52–72	VTESYAASLLQEQLGYINDKA (C-terminus)	154.66	3.40E−16	64
GtcpeA19	98–107	48–57	IAVSEGDAAR	51.02	7.90E−06	15
GtcpeA20	77–93	25–41	EYTGGLSNTQDDQMCVK	155.02	3.20E−16	84
	109–120	57–68	LLQENLSSMFGK (C-terminus)	153.03	5.00E−16	56
GtcpeA21	42–51	9–18	APVVTIFDHR	66.35	2.30E−07	176

The table shows the tryptic peptides with unique sequences of the phycobiliproteins of G. theta that are detected in the Mascot database searches. The search parameters permitted a mass error of 5 ppm and one missed cleavage site. The expectation value corresponds to the E-value of a Blast search result, and expectation values <0.05 have a confidence of >95%