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. 2018 Jan 24;8:1474. doi: 10.1038/s41598-018-19874-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ad5 and Ad19a/64 vectors mediate transgene expression in permissive cells. (A) Schematic representation of AdV and MVA genomes indicating transgene insertion sites. (B) Western Blot analysis of transgene expression 48 hours post infection (hpi). HEK293 T cells were infected at a multiplicity of infection (MOI) of 10 with Ad5 or Ad19a/64 vectors expressing the genes IE-1, pp65, or GFP as indicated. Full-length blots are displayed in Supplementary Figure 1.