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. 2018 Jan 24;8:1474. doi: 10.1038/s41598-018-19874-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ad19a/64 vectors are capable of transducing a broad panel of human blood cells. (A) Gating strategy to discriminate monocytes (CD14+), B cells (CD19+), NK cells (CD3-/CD56+) and T cells (CD4+ or CD8+). (B) Human peripheral blood mononuclear cells (PBMCs) from 3 different donors were transduced at the indicated MOIs with Ad5-GFP or Ad19a/64-GFP. The amount of GFP positive cells in the indicated populations was determined by flow cytometry at 24 hpi. Bars represent the mean and standard deviation (SD) of values from all donors. Asterisks indicate p-values below 0.05 (Bonferroni-corrected Wilcoxon signed rank test) when comparing Ad5 or Ad19a/64 transduced cells in a given subset paired for each donor and MOI.