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. 2018 Jan 24;8:1474. doi: 10.1038/s41598-018-19874-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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T cell stimulation by virally transduced DCs correlates with transduction rates. MoDCs from 3 different HCMV seronegative blood donors were infected at varying MOIs with the indicated vectors expressing IE-1 or pp65. 24 hpi, antigen-specific, HLA-matched T cell clones were added at an effector/target ratio of 1:1. After 6 hours of co-cultivation in the presence of Brefeldin A (BFA), cells were stained for CD8 and intracellular IFNγ and subjected to flow cytometry analysis. (A) Panel a shows the gating strategy employed to identify CD8/IFNγ positive cells. (B) The fraction of cells co-expressing CD8 and IFNγ is displayed as the mean and standard deviation of values from all donors.