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. 2018 Jan 24;8:1474. doi: 10.1038/s41598-018-19874-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MVA causes the highest amount of cell death in dendritic cells. MoDCs from 3 different HCMV seronegative blood donors were transduced at varying MOIs with the indicated vectors. 24 or 48 hpi, cells were stained with AnnexinV-APC and 7AAD before flow cytometric analysis. Panel A shows representative plots 48 hours after transduction with the indicated vectors at MOI 100. The results from all donors are summarized in (B). Bars show the mean and standard deviation (SD) of values from all donors (nd: not determined). Asterisks indicate conditions where the fraction of viable (double negative) cells was significantly (p < 0.05, Bonferroni-corrected t-test) reduced as compared to the uninfected control.