Figure 2.
Preparation and characterization of R2c@(BSA)2. (a) Preparation of R2c@(BSA)2. In R2c@(BSA)2, dimeric BSA was estimated to contain approximately ten R2c molecules. Relatively large cavities in dimeric BSA (PDB code: 4F5S), which were calculated by PyMOL, are highlighted in blue. (b) Typical gel-filtration chromatographs on Sephadex G-100. Elution profiles of R2c@(BSA)2 (top panel: red, ~280 nm; here, absorbance of R2c at ~280 nm was removed in order to extract the absorbance of BSA, bottom panel: blue, ~680 nm). (c) Electronic absorption spectra of R2c@(BSA)2 (top panel: black), R2c (bottom panel: blue) and BSA (bottom panel: red).