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. 2018 Jan 24;8:1533. doi: 10.1038/s41598-018-20010-2

Figure 5.

Figure 5

Secondary structure models of 5′-terminal regions of two mRNA constructs in which hairpins G56-C169 and U180-A218 were mutated and kinetics of their in vitro translation followed by a luciferase reporter gene assay. (a) Secondary structure models of the 5′UTRs of constructs: P1-Δ40p53(Δ57) and P1-Δ40p53(ΔHDM2). The predicted ΔG values for 5′UTR of each construct and for selected structural motifs are indicated. Parts of the 5′-terminal region of p53 mRNA are denoted as in Fig. 3. (b) The relative luciferase activity (RLU) was measured during translation of each mRNA construct in 1 s periods with 9-second intervals, for 16 minutes. The data illustrate approximate values of maximal translation efficiency for each construct.