Table 1.

Comparison of full-translation time and maximal luminescence activity for each mRNA construct.

mRNA construct	Full-translation time [min:s]	Maximal luminescence activity [%]
P0-p53	07:08 ± 00:16	33 ± 5
P1-p53	05:46 ± 00:02	300 ± 30
P0-Δ40p53(gUG2)	08:31 ± 00:10	60 ± 12
P1-Δ40p53(gUG2)	08:14 ± 00:09	69 ± 11
P0-Δ40p53	07:35 ± 00:15	33 ± 0.3
P1-Δ40p53	06:58 ± 00:14	100
P1-Δ40p53(L)	07:35 ± 00:08	169 ± 10
P1-Δ40p53(S)	08:00 ± 00:03	90 ± 4
P1-Δ40p53(Δ57)	07:57 ± 00:04	237 ± 9
P1-Δ40p53(ΔHDM2)	08:58 ± 00:04	58 ± 9

Full-translation time with standard deviation was calculated for each construct based on three independent experiments. The maximal luminescence activity obtained for each construct was normalized to the value obtained for the P1-Δ40p53 variant and standard deviations are indicated in the table.