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. 2018 Jan 24;8:1531. doi: 10.1038/s41598-018-19930-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Underestimation of radiation induced growth inhibition by MTT assay. NIH/3T3 (Ai–Aiii), Raw264.7 (Bi–Biii), HEK-293(Ci–Ciii), HeLa (Di–Diii), A549 (Ei–Eiii), MCF-7(Fi–Fiii) and MDAMB-231(Gi–Giii) were analyzed using MTT assay (Ai–Gi) and cell number estimation (N_t/N₀, Aii–Gii) at 5 Gy radiation dose. Further MTT values (ΔOD) were normalized with its respective cell number to quantify the metabolic viability/cell (Aiii to Giii, derived information) and presented as fold change with respect to control at different time intervals. Radiation induced growth inhibition (% value) for both MTT and cell number quantified and mentioned at 48 hours (Graph inset). Star shows the statistical significance of change between the groups. Data are expressed as mean ± SD (n = 4) *p < 0.05 vs. unirradiated cells.