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. 2018 Jan 24;8:1531. doi: 10.1038/s41598-018-19930-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Radiation increases mitochondrial mass per cell and enhances formazan formation: The mitochondrial mass was analyzed by staining cells with MitoTracker Green FM (100 nM; 20 min) at indicated time points. Graphs Ai- Gi (cell lines as described in Fig. 1) showing mean fluorescence intensity (MFI), presented as fold change with respect to control. (Aii–Gii) The Formazan formed per cell was quantified spectro-photometrically and presented as fold change of control at respective time points in different cell lines. (Aiii–Giii) Photomicrograph showing formazan accumulation (at 20X objective), in control and irradiated cells captured after 2 hrs of MTT incubation at 24 hrs post irradiation in different cell lines. Zoom image of one irradiated cell is highlighted to show the enhanced punctuated formazan deposition. Data are presented as mean ± SD (n = 4) *p < 0.05 vs. untreated control cells.