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. 2018 Jan 24;8:1531. doi: 10.1038/s41598-018-19930-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Radiation induces hyperactivation of mitochondria: (A) Photomicrographs showing mitochondrial membrane potential (MMP) in HeLa and MDA-MB-231 cells stained with TMRM (5 nM/ml; 30 min; 37 °C) at 24 hrs post irradiation. Radiation induced changes in mitochondrial MMP was quantified by DiOC6 (B) and superoxide radical was quantified using MitoSox (C) in HeLa and MDA-MB-231 cells using flow cytometer and presented as MFI fold change at indicated time points with respect to control. Star shows the statistical significance of change between the indicated groups. Data are expressed as mean ± SD from triplicates. *p < 0.05 vs. unirradiated control.