Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 24;8:1479. doi: 10.1038/s41598-018-19917-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

PHB2 knockdown promotes RD cell differentiation. (a) Detection of myogenin by Western blot. RD cells were transfected with siRNA and the growth medium was replaced 6 hours later. The next day, the medium was replaced with the differentiation medium (2% calf serum, H-DMEM). Cell extracts were subjected to immunoblotting with indicated antibodies, and GAPDH was detected as a loading control. Full-length blots are presented in Supplementary Fig. S1. (b) Immunofluorescence staining of myogenin in RD cells. Forty-eight hours after transfection, RD cells were fixed and subjected to immunostaining for myogenin (red). The nuclei of cells were counterstained with DAPI (blue). Scale bar 200 μm. Results are representative of three independent experiments. (c) Quantitative analysis of myogenin in panel B. Data are presented as the mean ± S.D. (n = 3), *P < 0.05, **P < 0.01, ***P < 0.001, two-tailed Student’s t test.