Table 1.
PCR primers used in this study.
| Primers | Targeted genes | Sequences (5′-3′) | Annealing temperature (°C) | Amplicon size (bp) | Reference |
|---|---|---|---|---|---|
| tetA-FW | tetA | GCGCGATCTGGTTCACTCG | 61 | 164 | 57 |
| tetA-RV | AGTCGACAGYRGCGCCGGC | ||||
| tetB-FW | tetB | TACGTGAATTTATTGCTTCGG | 59 | 206 | 58 |
| tetB-RV | ATACAGCATCCAAAGCGCAC | ||||
| tetC-FW | tetC | GCGGGATATCGTCCATTCCG | 68 | 207 | 59 |
| tetC-RV | GCGTAGAGGATCCACAGGACG | ||||
| tetD-FW | tetD | GGAATATCTCCCGGAAGCGG | 68 | 187 | 57 |
| tetD-RV | CACATTGGACAGTGCCAGCAG | ||||
| tetE-FW | tetE | GTTATTACGGGAGTTTGTTGG | 61 | 199 | 57 |
| tetE-RV | AATACAACACCCACACTACGC | ||||
| tetG-FW | tetG | GCAGAGCAGGTCGCTGG | 65 | 134 | 59 |
| tetG-RV | CCYGCAAGAGAAGCCAGAAG | ||||
| tetH-FW | tetH | CAGTGAAAATTCACTGGCAAC | 61 | 185 | 57 |
| tetH-RV | ATCCAAAGTGTGGTTGAGAAT | ||||
| tetJ-FW | tetJ | CGAAAACAGACTCGCCAATC | 61 | 184 | 57 |
| tetJ-RV | TCCATAATGAGGTGGGGC | ||||
| tetK-FW | tetK | TCGATAGGAACAGCAGTA | 55 | 169 | 60 |
| tetK-RV | CAGCAGATCCTACTCCTT | ||||
| tetL-FW | tetL | TCGTTAGCGTGCTGTCATTC | 55 | 267 | 60 |
| tetL-RV | GTATCCCACCAATGTAGCCG | ||||
| tetV-FW | tetV | GCCTACGGTTTCATCCTGGC | 65 | 351 | 61 |
| tetV-RV | CGAGACCACCTTCGACAGCG | ||||
| tetY-FW | tetY | ATTTGTACCGGCAGAGCAAAC | 68 | 181 | 57 |
| tetY-RV | GGCGCTGCCGCCATTATGC | ||||
| tetZ-FW | tetZ | CCTTCTCGACCAGGTCGG | 61 | 204 | 57 |
| tetZ-RV | ACCCACAGCGTGTCCGTC | ||||
| tetA(P)-FW | tetA(P) | CTTGGATTGCGGAAGAAGAG | 55 | 676 | 60 |
| tetA(P)-RV | ATATGCCCATTTAACCACGC | ||||
| tet30-FW | tet30 | CATCTTGGTCGAGGTGACTGG | 68 | 210 | 57 |
| tet30-RV | ACGAGCACCCAGCCGAGC | ||||
| tet31-FW | tet31 | CAATCACGCCCAAAAGAA | 53 | 564 | 62 |
| tet31-RV | TGTGCCATCCCAGTTTGT | ||||
| tet33-FW | tet33 | ATGCGGTTCCGCTGAA | 54 | 784 | 63 |
| tet33-RV | GGAAAATGCGTCAGTGACAA | ||||
| tet35-FW | tet35 | ATGCGCAAGACCGTCCTAC | 54 | 64 | |
| tet35-RV | CACACACTAGTAACGGTCGAA | ||||
| tet38-FW | tet38 | ATGAATGTTGAATATTCTAA | 42 | 106 | 65 |
| tet38-RV | TGGCTACAGAAATCAAT | ||||
| tet39-FW | tet39 | CTCCTTCTCTATTGTGGCTA | 47 | 701 | 66 |
| tet39-RV | CACTAATACCTCTGGACATCA | ||||
| tet40-FW | tet40 | CGGAGGAAGAGGACAAACCC | 56 | 446 | 67 |
| tet40-RV | TAAGCCGCTGCCGATAAGAC | ||||
| tet41-FW | tet41 | AATGCGATCAATTTCCGCCG | 55 | 166 | This study |
| tet41-RV | CGGCGAACAGCAGATTAACG | ||||
| tet42-FW | tet42 | TCTCGAGGATCACGAACCCT | 55 | 128 | This study |
| tet42-RV | ACTGGGACTCGATACACCCA | ||||
| tet45-FW | tet45 | GCTGAGCCATCCACTCATTT | 63 | 107 | 68 |
| tet45-RV | TTTCCTCTTGAGCGTTTATGC | ||||
| tetAB(46)-FW | tetAB(46) | GCTTCTTGGACCTTGACGGA | 55 | 580 | This study |
| tetAB(46)-RV | GTTCCTGACTCATGGCCACA | ||||
| tet47-FW | tet47 | GCGTTTGGCGTGGGTTTAAT | 55 | 627 | This study |
| tet47-RV | GACCCCTGTGGCATTGGTTA | ||||
| tcr3-FW | tcr3 | CGCTCAGTTCGACAAGACCT | 54 | 399 | This study |
| tcr3-RV | GTCTCCATCGAGTTCGCCAT | ||||
| otrB-FW | otrB | CCGACATCTACGGGCGCAAGC | 55 | 947 | 69 |
| otrB-RV | GGTGATGACGGTCTGGGACAG | ||||
| otrC-FW | otrC | ATGAAGTTCCGCCGAATGNA | 55 | 1860 | 70 |
| otrC-RV | TCAGGTCTTCTTGCGGAACTT | ||||
| tetM-FW | tetM | ACAGAAAGCTTATTATATAAC | 55 | 171 | 59 |
| tetM-RV | TGGCGTGTCTATGATGTTCAC | ||||
| tetO-FW | tetO | ACGGARAGTTTATTGTATACC | 60 | 171 | 59 |
| tetO-RV | TGGCGTATCTATAATGTTGAC | ||||
| tetQ-FW | tetQ | AGAATCTGCTGTTTGCCAGTG | 56 | 169 | 59 |
| tetQ-RV | CGGAGTGTCAATGATATTGCA | ||||
| tetS-FW | tetS | GAAAGCTTACTATACAGTAGC | 50 | 169 | 59 |
| tetS-RV | AGGAGTATCTACAATATTTAC | ||||
| tetT-FW | tetT | AAGGTTTATTATATAAAAGTG | 46 | 169 | 71 |
| tetT-RV | AGGTGTATCTATGATATTTAC | ||||
| tetW-FW | tetW | GAGAGCCTGCTATATGCCAGC | 64 | 168 | 59 |
| tetW-RV | GGGCGTATCCACAATGTTAAC | ||||
| tetB(P)-FW | tetB(P) | AAAACTTATTATATTATAGTG | 46 | 169 | 59 |
| tetB(P)-RV | TGGAGTATCAATAATATTCAC | ||||
| tet32-FW | tet32 | GAACCAGATGCTGCTCTT | 57 | 620 | 72 |
| tet32-RV | CATAGCCACGCCCACATGAT | ||||
| tet36-FW | tet36 | TTTCTGGCAGAGGTAGAACG | 57 | 250 | 73 |
| tet36-RV | TTAATTCCTTGCCTTCAACG | ||||
| tet44-FW | tet44 | AAAATAATCAACATTGGTATTCTTGCTCA | 56 | 1927 | 74 |
| tet44-RV | TAGTAACTTAATTTTCTTTTTTATTAAACATATGGCG | ||||
| otrA-FW | otrA | GAACACGTACTGACCGAGAAG | 55 | 778 | 69 |
| otrA-RV | CAGAAGTAGTTGTGCGTCCG | ||||
| tetX-FW | tetX | GAAAGAGACAACGACCGAGAG | 56.5 | 131 | 75 |
| tetX-RV | ACACCCATTGGTAAGGCTAAG | ||||
| tet34-FW | tet34 | ATACGGGGATGCAAACTTCA | 53 | 729 | 63 |
| tet34-RV | ACGAGTGAGCTCTGATGTCTCTT | ||||
| tet37-FW | tet37 | ATGGTTCGCTATTACTCTAAC | 45 | 177 | 76 |
| tet37-RV | ATCAGTCTCATATTTCGACA | ||||
| tetU-FW | tetU | ATGCAGCTAAGACGTGGC | 54 | 317 | 77 |
| tetU-RV | TTATTCGGTATCACTTCTCTGTC |