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. 2018 Jan 24;8:1535. doi: 10.1038/s41598-018-19729-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of 9t18:1 and 11t18:1 on cytotoxicity of leptin-treated or non-treated HUVECs. (A) The effect of 9t18:1 and 11t18:1 on viability of leptin-treated or non-treated HUVECs. HUVECs were pretreated with or without leptin (75 nmol/L) for 24 h and cultured with 11t18:1(25, 50, 100 μmol/L) for 24 h. (B) The effect of 9t18:1on viability of 9c11t-CLA-treated or non-treated HUVECs. HUVECs were pretreated with or without 9c11tCLA (5, 25, 50 μmol/L) for 24 h and cultured with 9t18:1 (100 μmol/L) for 24 h. (C) The effect of 11t18:1 + leptin on viability of 9c11t-CLA -treated or non-treated HUVECs. HUVECs were treated or non-treated with 9c11tCLA (5, 25, 50 μmol/L) and then cultured with the group of 11t18:1 (100 μmol/L) + leptin (75 nmol/L) for 24 h. Data were expressed as the mean of three individual experiments ± SD. Values labeled with different letters in each set indicate significant differences (p < 0.05).