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. 2018 Jan 24;8:1490. doi: 10.1038/s41598-018-19969-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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GFP labelling before and after optic nerve transection. In vivo fluorescence CLSO images from the same animal and retinal region, but at different time points, following optic nerve transection. (a,b) 5 weeks post-injection of the AAV2-DCX-GFP vector to label RGCs and (c,d) 14 days post-optic nerve transection to demonstrate the effects of RGC death on GFP labelling. Panels b and d are higher magnification views of the respective regions marked by boxes in panels a and c. Arrows indicate examples of a cell that has lost all fluorescence (red) and a cell with diminished fluorescence (yellow).