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. 2018 Jan 24;8:1515. doi: 10.1038/s41598-018-19894-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of MP on bone mechanical integrity: (a) A right shift was observed in the force-displacement curves of male and female HD MP rats. Male LD and HD MP rats showed a significant decrease in (b) energy to failure, (c) stiffness, and (d) ultimate force relative to Water rats. No significant effects were observed in MP-treated female rats. (e) Failure force did not show significant differences in MP-treated male and female rats. (^!p < 0.05 Water vs HD, ^!!p < 0.01 Water vs HD, ^‡p < 0.05 HD PF vs HD, ^‡‡p < 0.01 HD PF vs HD).