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| Primer | GenBank number | Sequence (5′–3′) | Base number | Annealing (°C) |
|---|---|---|---|---|
| Amylase-F | NM_001035016 | GAAATGGCCGTGTGACAGAATTTA | 24 | 64.3 |
| Amylase-R | ACAAAGACAAGTGCCCTGTCAGAA | 24 | ||
| Trypsin-F | NM_001113727 | TGTCTGCGGCTCACTGCTAC | 20 | 62.7 |
| Trypsin-R | GCTGGGATGGACGATACTCTTG | 22 | ||
| Lipase-F | NM_001205820 | GTGGAAGCAAATGATGGACAAG | 22 | 61.8 |
| Lipase-R | TGGGTTGAGGGTGAGCAGA | 19 | ||
| 18S rRNA-F | NR_036642 | ACCCATTCGAACGTCTGCCCTATT | 24 | 61.2 |
| 18S rRNA-R | TCCTTGGATGTGGTAGCCGTTTCT | 24 |
