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. 2018 Jan 23;9(1):e02284-17. doi: 10.1128/mBio.02284-17

FIG 1 .

FIG 1 

Evaluation of ELISA. Group 1 HA stalk antigen coated onto the wells of an ELISA plate maintained important conformational epitopes. (A to C) ELISA binding of group 1 HA stalk-binding monoclonal antibodies (in picograms per 50 μl) was measured using serial dilutions of monoclonal antibodies CR6261 (A), C179 (B), and 70-1F02 (C). (D) H1 HA globular head-binding monoclonal antibody EM4CO4 was used as a negative control. Anti-HA stalk serum titers measured from patient samples in this study showed strong positive correlation with the level of inhibition to potent group 1 HA stalk-binding monoclonal antibodies. Samples were ranked by the anti-HA stalk antibody titer and the percent inhibition to stalk-binding monoclonal antibodies for Spearman’s rank correlation analysis. (E to G) Increasing anti-HA stalk titer showed a strong and significant tendency of increasing inhibition of CR6261 (E), C179 (F), and 70-1F02 (G) binding.