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. 2017 Nov 12;7:57–70. doi: 10.1016/j.molmet.2017.10.014

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© 2017 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

VPS13C levels increase during BA differentiation. Brown pre-adipocytes were grown in cell culture and harvested at 1–6 days after differentiation. A) protein levels (n = 4) and B) VPS13C mRNA (n = 3) were measured by Western blot and qt-PCR respectively (ND = non-differentiated). C) BAs Postnuclear (PN), cytosol (cyto), LD, membrane (memb), mitochondrial (mito) and nuclear (nuclei) fractions were collected at 2 and 4 days after differentiation. VPS13C was detected by Western Blot. D) Immunofluorescence staining of VPS13C in BAs at 0–4 days after induction of differentiation. VPS13C = green, lipids stained with LipidTOX deep red.